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A culture system for <i>Artemia, Daphnia</i>, and other invertebrates, with continuous separation of the larvae OMA
Sorgeloos, P.; Persoone, G..
An easily constructed apparatus is described in which adult invertebrates can be reared with automatic feeding and with automatic and continuous separation of the offspring from the adults.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Culture media; Larvae.
Ano: 1973 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3471
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A simple method for subsampling zooplankton OMA
Persoone, G..
A simple and accurate method for subsampling zooplankton suspensions is described for which one only needs graduated plastic syringes and penicillin vials with a rubber stopper.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sampling; Sampling methods; Zooplankton.
Ano: 1972 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3455
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A simple volumeter for small invertebrates OMA
Persoone, G..
The displacement technique for measuring the volume of plankton catches is well established, and a number of papers have been published describing suitable apparatus for this purpose including those by Frolander (1957), and Yentsch & Hebard (1957). Gnanamuthu (1952) reviews several papers dealing with equipment for measuring the volume of small aquatic animals and discusses his own volumeter. The apparatus here described employs a water column and is, therefore, more convenient to handle than previous designs which used a mercury column. It is inexpensive and simple to construct, and will measure to 0.01 or even 0.005 ccm. We have found it most useful in quickly measuring the volume of polychaetes or oligochaetes, small bivalves, small crustaceans, etc.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Instruments; Marine invertebrates.
Ano: 1971 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3430
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Air-lift pumps and the effect of mixing on algal growth OMA
Persoone, G.; Morales, J.; Verlet, H.; De Pauw, N..
Since turbulence is an important factor for the growth of microscopic algae, the effect of mixing intensity and mixing time, has been assessed by means of air-lift pumps as an alternative technology for the agitation of algal cultures. Batch culture experiments in enriched sea water were carried out indoors with <i>Chlorella</i> in 100 liter tanks and outdoors with natural phytoplankton populations in 70 m³ tanks. The respective culturing depths in these tanks were 25 and 180 cm. The role of the mixing was also evaluated against that of the pH regulation of the cultures. Mixing with air-lift pumps increased the algal growth rate significantly in comparison to non-agitated cultures. The yields were also much higher (up to 30%) in mixed than in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Algae culture.
Ano: 1980 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3692
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An improved separator box for <i>Artemia</i> nauplii and other phototactic invertebrates OMA
Persoone, G.; Sorgeloos, P..
1. A simple cylindrical hatching and separator box is described for <i>Artemia salina</i>nauplii (and other phototactic invertebrates), in which the "corner seeking effect" responsible for poor separation is avoided, and from which nauplii can be recovered very conveniently. 2. Separation is achieved by partly unscrewing an internal and closed cylinder from its fixed counter-part glued to the bottom of the box, till two slits are freed through which the nauplii migrate from the darkened internal hatching cylinder into the illuminated exterior compartment. Rescrewing the former cylinder separates the larvae from debris and unhatched eggs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hatching; Instruments; Nauplii; Phototaxis; Artemia.
Ano: 1972 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3460
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Assessment of ammonia toxicity in contaminated sediments in the upper Scheldt (Belgium): the development and application of toxicity identification evaluation procedures OMA
Van Sprang, P.; Janssen, C.R.; Sabayasachi, M.; Benijts, F.; Persoone, G..
The toxicity of sediment pore waters from 4 sites in the Upper Scheldt war characterized using modified Toxicity Identification Evaluation (TIE) procedures The samples from all locations exhibited acute toxicity to the freshwater crustacean Thamnocephalus platyurus. The IIE approach was conducted using an enlarged battery of fractionation tests. Toxicity was removed or reduced significantly by the cation exchange resins and the air-stripping test at pH 11. Additionally, increased toxicity war observed at higher pH while a reduced toxicity war found at a decreased pH. From these TIE studies, ammonia war identified as the dominant toxic agent.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metals; River; Toxicants; Water.
Ano: 1996 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=7459
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Contributions à l'étude des bactéries marines du littoral belge: 1. Un appareil simple pour détacher et obtenir en suspension les bactéries contaminant des surfaces OMA
Persoone, G..
Description of a simple aparatus to remove and suspend bacteria contaminating surfaces.
Tipo: Info:eu-repo/semantics/article
Ano: 1964 URL: http://www.vliz.be/imisdocs/publications/294267.pdf
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Contributions à l'étude des bactéries marines du littoral belge: II. Comparaison de plusieurs méthodes pour détacher et obtenir en suspension les bactéries contaminant des surfaces OMA
Persoone, G..
Comparison of different methods to remove and to suspend bacteria which contaminate surfaces.The waterpressure and suction-rinsing method gave most satisfactory results as to the percentage of removed organisms and the number of them that we could bring into culture on agar-media.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria ANE; Belgium; Belgian Coast.
Ano: 1965 URL: http://www.vliz.be/imisdocs/publications/282301.pdf
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Contributions à l'étude des bactéries marines du littoral belge: III. Milieux de culture et ensemencements OMA
Persoone, G..
In order to detect a suitable medium for the enumeration of marine bacteria, several culture media were compared by two methods of plating: « pour plates » after Koch, and « spread plates ».The optimum period of incubation to get a maximum number of colonies on the dishes was determined.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria ANE; Belgium; Belgian Coast.
Ano: 1966 URL: http://www.vliz.be/imisdocs/publications/289062.pdf
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Contributions à l'étude des bactéries marines du littoral belge: IV. Recherche quantitative de la richesse microbienne de l'eau du bassin de chasse d'Ostende par lecture directe sur membranes filtrantes OMA
Persoone, G.; De Pauw, N..
In a series of bi-mensual samplings in 1966, the method of direct counting on membrane filters has been used to determine the quantity of bacteria present in the water of the Sluice-dock in Ostend (Belgium) as a possible important food-stock for oysters which are reared there.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria Community composition Filters Membranes Oyster culture Quantitative distribution Shallow water ANE; Belgium; Belgian Coast ANE; Belgium; Oostende; Sluice Dock.
Ano: 1968 URL: http://www.vliz.be/imisdocs/publications/294746.pdf
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De pollutie van de haven van Oostende en haar gevolgen op de reproductie van de platte oester (Ostrea edulis L.) in de Spuikom (extended abstract) OMA
Persoone, G..
Tipo: Info:eu-repo/semantics/article
Ano: 1971 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=7918
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Decapsulation of <i>Artemia</i> cysts: a simple technique for the improvement of the use of brine shrimp in aquaculture OMA
Sorgeloos, P.; Bossuyt, E.; Laviña, E.; Baeza-Mesa, M.; Persoone, G..
Although it is a common practice in different disciplines of fundamental research on the brine shrimp, and despite the very interesting applications that it offers for the use of <i>Artemia</i> in aquaculture, the "decapsulation" technique, which removes the outer layer of the cyst shell of <i>Artemia</i> , is not known to shrimp and fish aquaculturists. The present paper describes the technology developed by the authors for the routine decapsulation of <i>Artemia</i> cysts. The advantage which result from the use of decapsulated cysts in aquacultural hatcheries are discussed.
Tipo: Info:eu-repo/semantics/article
Ano: 1977 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3572
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Determination of the toxicity of four heavy metal compounds and three carcinogens using two marine nematode species, <i>Monhystera microphthalma</i> and <i>Diplolaimelloides bruciei</i> OMA
Bogaert, T.; Samoiloff, M.R.; Persoone, G..
Bioassays with two marine nematode species were used to determine the toxicity of four heavy metal compounds and three carcinogens. The test using the marine species <i>Diplolaimelloides bruciei</i> is more sensitive than the existing nematode test using the freshwater nematode <i>Panagrellus redivivus</i> for mercury chloride and 2-acetamidofluorene, but less sensitive for methylmercury chloride, phenacetin, and 4-aminobiphenyl. The <i>Monhystera microphthalma</i> test is less sensitive than the nematode test with <i>P. revivus</i> for mercury chloride, selenium oxide, and cesium chloride. The range between the concentrations at which no toxic effects are observed and the concentrations at which 100%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carcinogens Heavy metals Toxicity Diplolaimelloides bruciei Monhystera microphtalma de Man; 1880 Nematoda [Nematodes].
Ano: 1984 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3262
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Ecology of fouling on submerged surfaces in a polluted harbour OMA
Persoone, G..
Growth on submerged substrates (fouling or "Aufwuchs") has already been the object of numerous studies especially with regard to its economic implications. The scarcity of investigations on the influence of pollution on development and evolution of the fouling-biocoenosis is surprising. As far as we know the smaller fauna and flora in this particular situation have never been studied quantitatively. Therefore, in 1963, 1964 and 1965, a number of experiments were carried out in the harbour of Ostend (Belgium) on submerged substrates of different nature, in order to study the following less known aspects of fouling: a) the successive stages in the development of fouling, from bacteria to macro-organisms. b) the influence of several factors on its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fouling Harbours Marine pollution ANE; Belgium; Oostende Harbour.
Ano: 1971 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3431
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Ecotoxicological testing for the marine environment: report on an international Symposium held in Belgium in 1983 OMA
Persoone, G.; Jaspers, E.; Claus, C..
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ecotoxicology; Marine environment; Toxicity tests.
Ano: 1986 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3224
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Establishment of a test guideline for the evaluation of fish tainting OMA
Poels, C.L.M.; Fischer, R; Fukawa, K.; Howgate, P.; Maddock, B.G.; Persoone, G.; Stephenson, R.R.; Bontinck, W.J..
A test guideline has been established for determining the potential of a chemical to taint seafood. This guideline, based on the exposure of fish and the evaluation of the imparted taint by a triangular test, has been evaluated in a small ring test in which five laboratories tested four chemicals with three fish species. The guideline also contains a procedure for estimating the loss of taint from the test species during a depuration phase in clean water. None of the following factors appeared to have a large or systematic effect on the evaluation of the potential to cause taint: the performing laboratory; type of water used (sea- or freshwater); fish species; evaluation by flavour or by odour; method of preparation and presentation of samples for sensory...
Tipo: Info:eu-repo/semantics/article
Ano: 1988 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3155
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General aspects of the ecology and biogeography of <i>Artemia</i> OMA
Persoone, G.; Sorgeloos, P..
Tipo: Info:eu-repo/semantics/article Palavras-chave: Autecology; Biogeography; Artemia.
Ano: 1980 URL: http://www.vliz.be/imisdocs/publications/260376.pdf
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Hazard prediction and relevance of laboratory-scale studies OMA
Persoone, G..
Predictive hazard assessment of chemicals, pure, in mixtures, or as wastes, is based on the comparison of exposure to effect concentrations. Experimental determination of no-effect levels can be carried out at various levels of biological organisation: within the organism (suborganismal), on individual species (organismal), or with biological communities (supraorganismal). The difficulty of predictive effect analysis consists of relating endpoints determined at various levels of biological organisation to the impact on structure and/or function impairment of natural ecosystems. Examples are given of effect-extrapolations which have been validated at different levels, and which carry promising predictive values.
Tipo: Info:eu-repo/semantics/article
Ano: 1988 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3154
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Influence de quelques facteurs écologiques sur la vitesse de reproduction du cilié hypotriche <i>Euplotes vannus</i> Muller OMA
Persoone, G.; Deplaecie, M..
During ecological investigations on the optimum culturing conditions of ciliates, we studied the influence of a number of abiotic factors on the reproductive potential of the hypotrichous ciliate <i>Euplotes vannus</i> Muller, such as the nature and the quantity of food, the temperature and the salinity. The experiments were run in petri dishes of 5 cm diameter containing 5 ml of food suspension, and were always started with conjugants. The microphagous <i>Euplotes vannus</i> thrives on a variety of food media. Excellent results were observed on homogenized bakers yeast, i.e. 10.000-20.000 animals in 5 ml of food suspension. In good culturing conditions the stationary phase was reached in 7 days, which implicates a mean rate of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reproduction Euplotes vannus ANE; Belgium; Oostende Harbour.
Ano: 1972 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3468
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Kennen Sie die Lebensgeschichte von <i>Artemia salina</i>? OMA
Sorgeloos, P.; Persoone, G..
Tipo: Info:eu-repo/semantics/article Palavras-chave: Life history; Artemia salina.
Ano: 1972 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3437
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